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種屬 |
人 |
|
別稱 |
SK-Mel-5; SK MEL 5; SK.MEL.5; SK-MEL5; SKMel-5; SKMEL-5; SKMEL5; SKMel5; SKmel5; AA-Mel |
|
組織來源 |
皮膚 |
|
疾病 |
皮膚黑色素瘤 |
|
傳代比例/細(xì)胞消化 |
1:2傳代,消化2-3分鐘 |
|
完全培養(yǎng)基配置 |
MEM培養(yǎng)基；10%胎牛血清；1%雙抗 |
|
簡介 |
SK-MEL-5 is a melanoma cell line isolated from skin tissue obtained from a 24-year-old, White, female malignant melanoma patient. This cell line is a suitable transfection host and also serves as a source of target cells for the detection of melanoma-specific antibody in patients with the disease. This is one of a very extensive series of melanoma lines isolated by T. Takahashi and associates. |
|
形態(tài) |
星狀細(xì)胞樣 |
|
生長特征 |
貼壁生長 |
|
倍增時間 |
每周 2 至 3 次 |
|
抗原表達(dá) |
Blood Type O; Rh+; HLA A2, A11, B40, Bw16 |
|
致瘤性 |
Yes, in nude mice; forms malignant melanoma |
|
STR |
Amelogenin: X CSF1PO: 10,13 D13S317: 10,12 D16S539: 10,12 D5S818: 11,13 D7S820: 9,12 THO1: 6,9 TPOX: 11 vWA: 14,18 |
|
培養(yǎng)條件 |
氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度，培養(yǎng)箱濕度為70%-80%。 |
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凍存條件 |
凍存液：90%FBS，DMSO 10%, 或使用非程序凍存液：官網(wǎng)貨號JY-H040 |
|
保藏機構(gòu) |
ATCC; HTB-70 |
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產(chǎn)品使用 |
僅限于科學(xué)研究，不可作為動物或人類疾病的治療產(chǎn)品使用。 |
