種屬 |
小鼠 |
別稱 |
HL1 |
組織來源 |
小鼠心肌細胞 |
疾病 |
轉化細胞系 |
傳代比例/細胞消化 |
1 :2傳代 ,消化2-3分鐘 |
完全培養(yǎng)基配置 |
Claycomb Basal Medium培養(yǎng)基;10%胎牛血清 ;2mM Glutamax;1%Anti-Anti;100 μM Norepinephrine; |
簡介 |
HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion. |
形態(tài) |
心肌細胞樣 |
生長特征 |
貼壁生長 |
倍增時間 |
每周 2 至 3 次 |
培養(yǎng)條件 |
氣相 :空氣 ,95% ;二氧化碳 ,5%。 溫度 :37攝氏度 ,培養(yǎng)箱濕度為70%-80%。 |
凍存條件 |
凍存液 :90%FBS ,DMSO 10%, 或使用非程序凍存液 :官網(wǎng)貨號JY-H040 |
保藏機構 |
Millipore;SCC065 |
備注 |
該細胞貼壁性不牢,需使用Corning的cellbind細胞培養(yǎng)瓶,貨號是3289 ,或者用包被液包被的培養(yǎng)板應放在培養(yǎng)箱(37℃)下至24h以上,或者提前一天。 |
產(chǎn)品使用 |
僅限于科學研究 ,不可作為動物或人類疾病的治療產(chǎn)品使用。 |